Lab 4 Report Example | Topics and Well Written Essays - 750 words

4 - Lab Report Example In other words the more yeast put in the Hydrogen Peroxide the more violent the reaction; therefore, giving off more oxygen. This is because of the reaction theory (the rate of a reaction depends on how often and how hard the reacting particles collide with each other) Response to Question 3 As the concentration f the enzymes decreases, so does the speed and vigor of the reaction. This is evidenced from the low volume of oxygen produced in tube 3 with less enzyme concentration. As the enzyme concentration decreases, the rate of reaction also decreases because there are fewer enzymes present to break down the substrate. Response to Question 4 More enzyme activity is expected if the substrate concentration is decreased. When there are low concentrations of substrate there is a linear relationship between the reaction rate and substrate concentration. In these conditions the relation of catalase enzyme to hydrogen peroxide substrate molecules is high. Experiment 2: Effect of temperature on enzyme activity. Data Table 2: Effect of temperature on the production of gas Tube Temperature ?C Balloon diameter (cm) Refrigerator 6 5.0 Room temperature 23 12.5 Hot water 90 1.0 Response to Question 1 The enzyme in this experiment is catalase enzyme and hydrogen peroxide is the substrate. Response to Question 2 The rate of enzyme catalyzed reaction tends to  increase with the increasing temperature of reaction medium. Enzymes are known to be proteins, they are gradually denatured thus lose their activity at the temperatures beyond their optimum temperature. In this case, catalase enzyme’s maximum activity is around 37? degrees Celsius. At extremely low temperatures, the reactants move around slower because of having less kinetic energy. This means that collisions occurring between the substrate and catalase enzyme active site happen less resulting in reduced enzyme-substrate complexes, therefore, slow down the reaction. An increase in temperature causes the reactants to have more kinetic energy. They, therefore, end up moving around faster and the movements cause more collisions between the substrate and active site of the enzyme. Therefore, there shall be more enzyme-substrate complexes taking place hence speeding up the reaction. Temperatures above the optimum alter the shape of the active site of enzymes. This  causes the temperature to denature the enzyme. In effect, enzyme-substrates would not be created due to the substrate not fitting in the active site. Denaturing the enzyme would mean that the tertiary structure is altered, which would end up causing the reaction process to stop completely. Response to Question 3. Yes plants and animals have the enzymes that breakdown hydrogen peroxide. Response to Question 4 Maintaining an optimum temperature of about 37 degrees Celsius increases the enzyme activity. Response to Question 5. The presence of catalase enzyme in cells catalyses the toxic hydrogen peroxide to non toxic products that incl ude, water and Oxygen. Hydrogen Peroxide ----–catalase enzyme---- Water + Oxygen. Response to Question 6 Experiment to determine the optimal temperature for enzyme function The experiment would be set up as below. First, set up the water bath; secondly, place a thermometer in the water bath so that the proper temperature is maintained. 0-5degrees Celsius: 400mL beaker to be filled with water and ice 20-25 degrees Celsius: No water bath is required to maintain the room